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PAGE investigation of CANVAS pathogenic dAGGGA repeats. Migration pattern of dAGGGA repeats and of the reference oligonucleotides d(TGGGTT) n and d(TGGGT) n ( A ) in 100 mM KCl, detected by UV-shadowing; ( B ) in 100 mM KCl, detected by NMM and SYBR Safe staining; ( C ) in 100 mM LiCl, detected by SYBR Safe staining; ( E ) in 100 mM KCl, detected by ThT staining. Strand concentrations: 240/ n μM in gel ( A ), 24/ n μM in gels ( B, C, E ). Electrophoresis of gel ( A ) was carried out at room temperature, whereas gels ( B, C, E ) were run in a cold room. The symbol ”*” marks the major bands detected for d(AGGGA) 4 in gels ( A ) and ( E ) and the d(TGGGT) 8 band in gel ( E ). <t>DNA</t> <t>ladder</t> sizes: 10, 15, 20, 25, 35, 50, 75, 100, 150, 200, 300 bp. Sample buffer: 10 mM cacodylic acid, pH 7.2. ( D ) Schematic representation of the intermolecular G4 and duplex structures formed by d(AGGGA) 8,12,16 corresponding to the gel bands framed by red and blue rectangles, respectively, and schematic representation of putative bimolecular G4s that may be formed by d(AGGGA) 4 .
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PAGE investigation of CANVAS pathogenic dAGGGA repeats. Migration pattern of dAGGGA repeats and of the reference oligonucleotides d(TGGGTT) n and d(TGGGT) n ( A ) in 100 mM KCl, detected by UV-shadowing; ( B ) in 100 mM KCl, detected by NMM and SYBR Safe staining; ( C ) in 100 mM LiCl, detected by SYBR Safe staining; ( E ) in 100 mM KCl, detected by ThT staining. Strand concentrations: 240/ n μM in gel ( A ), 24/ n μM in gels ( B, C, E ). Electrophoresis of gel ( A ) was carried out at room temperature, whereas gels ( B, C, E ) were run in a cold room. The symbol ”*” marks the major bands detected for d(AGGGA) 4 in gels ( A ) and ( E ) and the d(TGGGT) 8 band in gel ( E ). <t>DNA</t> <t>ladder</t> sizes: 10, 15, 20, 25, 35, 50, 75, 100, 150, 200, 300 bp. Sample buffer: 10 mM cacodylic acid, pH 7.2. ( D ) Schematic representation of the intermolecular G4 and duplex structures formed by d(AGGGA) 8,12,16 corresponding to the gel bands framed by red and blue rectangles, respectively, and schematic representation of putative bimolecular G4s that may be formed by d(AGGGA) 4 .
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PAGE investigation of CANVAS pathogenic dAGGGA repeats. Migration pattern of dAGGGA repeats and of the reference oligonucleotides d(TGGGTT) n and d(TGGGT) n ( A ) in 100 mM KCl, detected by UV-shadowing; ( B ) in 100 mM KCl, detected by NMM and SYBR Safe staining; ( C ) in 100 mM LiCl, detected by SYBR Safe staining; ( E ) in 100 mM KCl, detected by ThT staining. Strand concentrations: 240/ n μM in gel ( A ), 24/ n μM in gels ( B, C, E ). Electrophoresis of gel ( A ) was carried out at room temperature, whereas gels ( B, C, E ) were run in a cold room. The symbol ”*” marks the major bands detected for d(AGGGA) 4 in gels ( A ) and ( E ) and the d(TGGGT) 8 band in gel ( E ). <t>DNA</t> <t>ladder</t> sizes: 10, 15, 20, 25, 35, 50, 75, 100, 150, 200, 300 bp. Sample buffer: 10 mM cacodylic acid, pH 7.2. ( D ) Schematic representation of the intermolecular G4 and duplex structures formed by d(AGGGA) 8,12,16 corresponding to the gel bands framed by red and blue rectangles, respectively, and schematic representation of putative bimolecular G4s that may be formed by d(AGGGA) 4 .
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PAGE investigation of CANVAS pathogenic dAGGGA repeats. Migration pattern of dAGGGA repeats and of the reference oligonucleotides d(TGGGTT) n and d(TGGGT) n ( A ) in 100 mM KCl, detected by UV-shadowing; ( B ) in 100 mM KCl, detected by NMM and SYBR Safe staining; ( C ) in 100 mM LiCl, detected by SYBR Safe staining; ( E ) in 100 mM KCl, detected by ThT staining. Strand concentrations: 240/ n μM in gel ( A ), 24/ n μM in gels ( B, C, E ). Electrophoresis of gel ( A ) was carried out at room temperature, whereas gels ( B, C, E ) were run in a cold room. The symbol ”*” marks the major bands detected for d(AGGGA) 4 in gels ( A ) and ( E ) and the d(TGGGT) 8 band in gel ( E ). <t>DNA</t> <t>ladder</t> sizes: 10, 15, 20, 25, 35, 50, 75, 100, 150, 200, 300 bp. Sample buffer: 10 mM cacodylic acid, pH 7.2. ( D ) Schematic representation of the intermolecular G4 and duplex structures formed by d(AGGGA) 8,12,16 corresponding to the gel bands framed by red and blue rectangles, respectively, and schematic representation of putative bimolecular G4s that may be formed by d(AGGGA) 4 .
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PAGE investigation of CANVAS pathogenic dAGGGA repeats. Migration pattern of dAGGGA repeats and of the reference oligonucleotides d(TGGGTT) n and d(TGGGT) n ( A ) in 100 mM KCl, detected by UV-shadowing; ( B ) in 100 mM KCl, detected by NMM and SYBR Safe staining; ( C ) in 100 mM LiCl, detected by SYBR Safe staining; ( E ) in 100 mM KCl, detected by ThT staining. Strand concentrations: 240/ n μM in gel ( A ), 24/ n μM in gels ( B, C, E ). Electrophoresis of gel ( A ) was carried out at room temperature, whereas gels ( B, C, E ) were run in a cold room. The symbol ”*” marks the major bands detected for d(AGGGA) 4 in gels ( A ) and ( E ) and the d(TGGGT) 8 band in gel ( E ). <t>DNA</t> <t>ladder</t> sizes: 10, 15, 20, 25, 35, 50, 75, 100, 150, 200, 300 bp. Sample buffer: 10 mM cacodylic acid, pH 7.2. ( D ) Schematic representation of the intermolecular G4 and duplex structures formed by d(AGGGA) 8,12,16 corresponding to the gel bands framed by red and blue rectangles, respectively, and schematic representation of putative bimolecular G4s that may be formed by d(AGGGA) 4 .
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Image Search Results


PAGE investigation of CANVAS pathogenic dAGGGA repeats. Migration pattern of dAGGGA repeats and of the reference oligonucleotides d(TGGGTT) n and d(TGGGT) n ( A ) in 100 mM KCl, detected by UV-shadowing; ( B ) in 100 mM KCl, detected by NMM and SYBR Safe staining; ( C ) in 100 mM LiCl, detected by SYBR Safe staining; ( E ) in 100 mM KCl, detected by ThT staining. Strand concentrations: 240/ n μM in gel ( A ), 24/ n μM in gels ( B, C, E ). Electrophoresis of gel ( A ) was carried out at room temperature, whereas gels ( B, C, E ) were run in a cold room. The symbol ”*” marks the major bands detected for d(AGGGA) 4 in gels ( A ) and ( E ) and the d(TGGGT) 8 band in gel ( E ). DNA ladder sizes: 10, 15, 20, 25, 35, 50, 75, 100, 150, 200, 300 bp. Sample buffer: 10 mM cacodylic acid, pH 7.2. ( D ) Schematic representation of the intermolecular G4 and duplex structures formed by d(AGGGA) 8,12,16 corresponding to the gel bands framed by red and blue rectangles, respectively, and schematic representation of putative bimolecular G4s that may be formed by d(AGGGA) 4 .

Journal: Nucleic Acids Research

Article Title: Pentanucleotide guanine-rich WGGGW repeats, including CANVAS AGGGA repeats, form a variety of noncanonical structures

doi: 10.1093/nar/gkag051

Figure Lengend Snippet: PAGE investigation of CANVAS pathogenic dAGGGA repeats. Migration pattern of dAGGGA repeats and of the reference oligonucleotides d(TGGGTT) n and d(TGGGT) n ( A ) in 100 mM KCl, detected by UV-shadowing; ( B ) in 100 mM KCl, detected by NMM and SYBR Safe staining; ( C ) in 100 mM LiCl, detected by SYBR Safe staining; ( E ) in 100 mM KCl, detected by ThT staining. Strand concentrations: 240/ n μM in gel ( A ), 24/ n μM in gels ( B, C, E ). Electrophoresis of gel ( A ) was carried out at room temperature, whereas gels ( B, C, E ) were run in a cold room. The symbol ”*” marks the major bands detected for d(AGGGA) 4 in gels ( A ) and ( E ) and the d(TGGGT) 8 band in gel ( E ). DNA ladder sizes: 10, 15, 20, 25, 35, 50, 75, 100, 150, 200, 300 bp. Sample buffer: 10 mM cacodylic acid, pH 7.2. ( D ) Schematic representation of the intermolecular G4 and duplex structures formed by d(AGGGA) 8,12,16 corresponding to the gel bands framed by red and blue rectangles, respectively, and schematic representation of putative bimolecular G4s that may be formed by d(AGGGA) 4 .

Article Snippet: As DNA ladder, we used Thermo ScientificTM GeneRuler Ultra Low Range DNA Ladder, ready-to-use.

Techniques: Migration, Staining, Electrophoresis